. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. 172 BIOLOGY AND TECHNIQUE in obliquely so as to avoid penetrating the abdominal wall and entering the peritoneum. In making intraperitoneal inoculations, great care must be exercised not to puncture the gut. This can be avoided by passing the needle first through the skin in an oblique direction, then turning it into a posi- tion more vertical to the abdomen and perforating the muscles and perito- neum by a very short and carefully executed stab. Intravenous inoculations in rabbits are
. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. 172 BIOLOGY AND TECHNIQUE in obliquely so as to avoid penetrating the abdominal wall and entering the peritoneum. In making intraperitoneal inoculations, great care must be exercised not to puncture the gut. This can be avoided by passing the needle first through the skin in an oblique direction, then turning it into a posi- tion more vertical to the abdomen and perforating the muscles and perito- neum by a very short and carefully executed stab. Intravenous inoculations in rabbits are made into the veins running along the outer margins of the ears. The hair over the ear is clipped and the animal held for a short time head downward so that the vessels of the head may fill with blood. An assistant holds the animal firmly in. Fig. —Rabbit Cage. a horizontal position, the operator grasp's the tip of the ears with the left hand, and carefully passes his needle into the vein in the direction as nearly as possible parallel to its course. (See Fig. 50.) Mice are usually inoculated under the skin near the base of the tail. They may be placed in a jar over which a cover of stiff wire-gauze is held. They are then grasped by the tail, by which they are drawn up between the side of the jar and the edge of the wire cover, so that the lower end of the back shall be easily accessible. The skin is then wiped with a piece of cotton dipped in carbolic solution and the needle is in- serted. Great care must be exercised to avoid passing the needle too close to the vertebral column. Mice are extremely delicate, and any injury to the spine usually causes immediate Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Hiss, Philip Hanson, 1868-1913; Zinsser, Hans, 1878-1940. joint author. New York and L
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