. Laboratory work in bacteriology. Bacteriology. 264 BACTERIOLOGY. Fig-. 42 is made use of. This apparatus can be readily im- provised out of an ordinary 250 measuring cylinder. Usually, bouillon cultures are employed for the pur- pose of injection. Occasionally, however, the bacterial growth is on a solid medium, like agar or potato. In that case, bouillon or sterile water should be introduced into the tube by means of a drawn out tube pipette (Fig. 61). The growth is then thoroughly stirred up in the liquid by means of the end of the pipette. The suspension is then drawn up into the tub
. Laboratory work in bacteriology. Bacteriology. 264 BACTERIOLOGY. Fig-. 42 is made use of. This apparatus can be readily im- provised out of an ordinary 250 measuring cylinder. Usually, bouillon cultures are employed for the pur- pose of injection. Occasionally, however, the bacterial growth is on a solid medium, like agar or potato. In that case, bouillon or sterile water should be introduced into the tube by means of a drawn out tube pipette (Fig. 61). The growth is then thoroughly stirred up in the liquid by means of the end of the pipette. The suspension is then drawn up into the tube, and transferred to a sterile Esmarch dish, or to a small sterile conical test-glass (Fig. 43). In the case of a bouillon culture, this is transferred directly to the sterile vessel. The neck of the tube, after withdrawal of the cotton Fig. 42. Apparatus for injecting large quantities of liquid. plug, must, of course, be heated in the flame before pouring out the culture. When injecting small animals care must be taken that the needle does not pass through the abdominal wall into the peritoneal cavity. A fold of the skin should be raised, and the needle inserted while the syringe is held in a position almost parallel to that of the body. A success- ful subcutaneous injection will show a swelling over the place of The injection is made most conveniently over the abdominal wall, inasmuch as the skin is softer there than elsewhere. The rabbit. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Novy, Frederick George, 1864- . Ann Arbor [Mich. ] G. Wahr
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