. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 456 R. H. EARTH, JR. AND WILLIAM J. BELL lated directly by the corpora allata (Roth and Earth, 1964; Barth, 1968; Engel- mann and Barth, 1968). The results of the female pheromone assay above indicate that the female herself is not receptive at the time when sex pheromone on the stir- face of her cuticle makes her attractive to males. In view of our interest in the sequence of reproductive events we continued to test a small number of active females daily for signs of receptivity. All six animals so tested showed antenna- ti
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 456 R. H. EARTH, JR. AND WILLIAM J. BELL lated directly by the corpora allata (Roth and Earth, 1964; Barth, 1968; Engel- mann and Barth, 1968). The results of the female pheromone assay above indicate that the female herself is not receptive at the time when sex pheromone on the stir- face of her cuticle makes her attractive to males. In view of our interest in the sequence of reproductive events we continued to test a small number of active females daily for signs of receptivity. All six animals so tested showed antenna- tion and mounting of displaying males within the two minute test period on the second day following appearance of pheromone on the cuticle. Thus females appear to reach a high level of sexual receptivity about 2 days after the onset of pheromone secretion. It may be noted that this is likely to be several days before the average onset date for pheromone secretion as determined by the filter paper assay. Herein lies the probable explanation for the accounts in the literature of mating in B. fiunigata females prior to the onset of pheromone production (Barth, 1962; Roth and Stay, 1962). z D HJ Q cn o u D 8OO - eoo - 4oo - 200 -. 12 16 2O DAYS AFTCR EMERGENCE 24 28 FIGURE 6. Changes in left colleterial gland glucoside concentration during the first pre- oviposition period. Each point represents the average glucoside units measured for the pooled glands of 5 females. 4. Colleterial gland activity The left colleterial gland of B. fumigata secretes the structural protein of which the ootheca is composed and a glucoside of the phenolic tanning agent (Stay and Roth, 1962). Synthesis and storage of the protein is indicated by a bluish green color and increase in width of the gland tubules. The appearance of color and increase in colleterial gland size was observed to occur between days 4 and 6. In addition to the glucoside with an absorption peak of 2770 A, the glands contained an unknown
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
