. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 314 D. A. ROBERTS ET AL. incubation temperature increased, the relative intensi- ties of bands representing newly synthesized proteins changed markedly, with bands of molecular mass corre- sponding to major classes of heat shock proteins showing the strongest increase in intensity (Fig. 2). The most prominent bands of heat-inducible protein were of ap- parent A/r 68-74 kDa and kDa—bands that we as- sume to represent heat shock proteins of the 70-kDa and 30-kDa classes. These bands were faint or absent at tem- peratures
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 314 D. A. ROBERTS ET AL. incubation temperature increased, the relative intensi- ties of bands representing newly synthesized proteins changed markedly, with bands of molecular mass corre- sponding to major classes of heat shock proteins showing the strongest increase in intensity (Fig. 2). The most prominent bands of heat-inducible protein were of ap- parent A/r 68-74 kDa and kDa—bands that we as- sume to represent heat shock proteins of the 70-kDa and 30-kDa classes. These bands were faint or absent at tem- peratures below 20°C, and exhibited a marked increase in intensity and width at temperatures of 23°C and higher (Fig. 2). The appearance of two strong bands with A/r values near 70 kDa indicated de novo synthesis of more than one hsp70 isoform (Fig. 2). Other protein bands, whose synthesis was not induced by heat, were consistently present in all samples and at all tempera- tures tested. Most notable were two bands of 39 and 46 kDa (Fig. 2). However, synthesis of these non-heat- induced proteins decreased at 25° and 28°C, tempera- tures at which hsp synthesis remained strong. Seasonal and tidal-height patterns in hsplO induction profiles. In February, in mussels collected from a mid- intertidal site, there was a significant increase in the rela- tive amount of hsp70 synthesis at 23°, 25°, and 28°C as compared to synthesis at lower temperatures (Fig. 3; AN- OVA, P < in all cases). Between 23° and 28°C there was a twofold increase in the relative amount of hsp70 synthesized, and synthesis levels at 28°C were about four times higher than at 10°, 17°, and 20°C (Fig. 3). The im- portance of normalizing hsp70 synthesis to the 46-kDa protein to determine induction temperatures is illus- trated by comparing data in Figures 2 and 3. Although Figure 2 appears to show an increase (induction) in hsp70 synthesis at 20°C, when the 70-kDa band is nor- malized to the 46-kDa band
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