. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. INTESTINAL FLORA OF TERMITES 89 Woody material obtained with termites from Dr. Cory was used to inoculate a tube at room temperature under aerobic conditions. Th material was composed of wood and digested material, but no loos soil. When examined 9 days later the paper at the liquid level showe^ a discoloration which was marked above liquid level. Green discolora-\ tion was prominent. One colony mm. in diameter produced a wine- colored discoloration. Transplants on silica-gel plates produced abundant growth. The silica-g
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. INTESTINAL FLORA OF TERMITES 89 Woody material obtained with termites from Dr. Cory was used to inoculate a tube at room temperature under aerobic conditions. Th material was composed of wood and digested material, but no loos soil. When examined 9 days later the paper at the liquid level showe^ a discoloration which was marked above liquid level. Green discolora-\ tion was prominent. One colony mm. in diameter produced a wine- colored discoloration. Transplants on silica-gel plates produced abundant growth. The silica-gel was made fundamentally according to the general formula of Winogradsky (1929). Seventy-five grams of potassium silicate were dissolved in 1000 cc. of distilled water. To this was added an equal quantity of HC1 of a specific gravity of Thirty cc. of mixture were placed in the petri dishes and set aside under cover for 24 hours. They were then immersed in running water for 24 hours. They were next washed four successive times for 24 hours each in large covered dishes of sterile distilled water. From the following inorganic salt solution (made up for 100 plates) 2 cc. were added to each silica-gel dish. KH2PO4 gram MgSO4 gram NaCl gram FeSO4 gram MnSO4 gram KNO;! grams CaCO- grams Distilled water 200 cc. The pH was adjusted to The petri dishes were then placed in an incubator at ° C. until excess moisture evaporated. Sterilized pieces of Whatman's filter paper were placed aseptically on silica-gel plates. Sterile covers were then placed over the plates. Stroke in- oculations were made. Termites received from Dr. Cory were transferred to clean petri dishes in which were placed filter paper and the tissue paper sent with the original shipment. This was being eaten by the termites. On March 8, pellets of termite excreta which had been dropped on the tissue paper were used to inoculate two nitrate-cellulose tubes and one silica-gel plat
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
