. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 240 W. B. JAECKLE AND D. T. MANAHAN Table I Changes in the amount oj dry organic' weight between oocytes (day 0) and ve/iger lan'ae (day 5,6 or 7), sampled al the end oj the larval life span (competent to settle), during the complete development o/"Haliotis rufescens Culture 1 Culture 2 Culture 3 Culture 4 Culture 5 Age Age Age Age Age (day) (day) (day) (day) (day) 0 ± 0 ± 0 ± 0 + 0 ± 7 1 . 1 7 ± 6 ± 6 ± 6 + 5 + () Percent dif
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 240 W. B. JAECKLE AND D. T. MANAHAN Table I Changes in the amount oj dry organic' weight between oocytes (day 0) and ve/iger lan'ae (day 5,6 or 7), sampled al the end oj the larval life span (competent to settle), during the complete development o/"Haliotis rufescens Culture 1 Culture 2 Culture 3 Culture 4 Culture 5 Age Age Age Age Age (day) (day) (day) (day) (day) 0 ± 0 ± 0 ± 0 + 0 ± 7 1 . 1 7 ± 6 ± 6 ± 6 + 5 + () Percent difference between beginning and end of each culture (+) (+) (+) (+) Cultures 1 -5 refer to batches of larvae reared from gametes obtained from five separate spawings. All data are presented as the mean dry organic weight per individual (jjg ± 1 standard error of the mean). The percent difference, given at the bottom of the table, represents the net change in do- organic weight for each culture. calculated as mm Hg larva 'h '. This depletion rate was converted to moles of oxygen consumed by calibrating the electrode relative to the amount of oxygen in isother- mal seawater, as determined by the Winkler titration method (Parsons et al,, 1984). Results Changes in weight during the lan'al life span Figure 1 shows the changes in dry organic weight, and ash weight, from an unfertilized oocyte (day 0) to a larva competent to settle (day 7). In this culture (Culture 1). and all others studied (see Table I, Cultures 1 to 5), there was a continual linear increase in ash weight during lar- val development. There was a statistically significant net increase in dry organic weight from the oocyte (day 0) to the newly formed veliger larva (day 2), as can be seen in Figure 1 (Variance ratio, VR = , F00,n[] = ). Similar increases during this period of development (day 0 to day 2) were seen in four other cultures. For Culture 1 (Fig. 1), there was a statisticall
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