. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 234 S. LAPEGUE ET Figure 1. Population collection sites and their ta\ononne status based on IftS gene analysis. The arrows indicate the predominant surface circulation patterns in this part of the Atlantic Ocean. See legend of Table 1 for details on the three PAR samples. same bay. In this second sample, two groups (PAR2 and PAR3) were selected on the basis of their size: PAR2 specimens described as "fast growers," and PAR3 speci- mens described as "slow ; Two other samples from Brazil were c
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 234 S. LAPEGUE ET Figure 1. Population collection sites and their ta\ononne status based on IftS gene analysis. The arrows indicate the predominant surface circulation patterns in this part of the Atlantic Ocean. See legend of Table 1 for details on the three PAR samples. same bay. In this second sample, two groups (PAR2 and PAR3) were selected on the basis of their size: PAR2 specimens described as "fast growers," and PAR3 speci- mens described as "slow ; Two other samples from Brazil were collected in 1999: in the Cananeia Bay (CAN). and near Salvador do Bahia (BAH). Putative C. guxcir samples were provided in 1999 from locations along the Senegalese coasts (ZIG. NOB. PIC. ALM. SOM, MBO, FAD), and specimens were taken from the Niger estuary (DAM), described as C. rhizophorae in Nigeria in 2000. Generally, the samples were collected on either mangrove roots or rocks; but in the Paraguana Bay. they were all sampled on rocks, and in French Guiana on mangrove roots. Table 1 summarizes the characteristics of these samples. Two animals from each of the populations SIN and NOB were chosen for karyological analysis, as they were initially thought to represent C. rhizophorae and C. XCIMII- respec- tively. Mitochondrial DNA </H<;'.S DNA extraction of gill fragments was performed either by a Chelex-based method, as described in Estoup ct til. (1996), or by a phenol/chloroform method, as described by Moore (1993). We amplified the 16S mitochondria! frag- ment (16SrDNA: the large subunit rRNA-coding gene) with primers described by Banks ft ul. (1993), according to the protocol detailed in Boudry et til. (1998). A first set of samples (two individuals from each of nine populations, as indicated in Table 1) was studied by se- quencing the mitochondria! 16S fragment. The PCR prod- ucts were then purified with a high pure PCR product purification kit (Boehringer-Mannhe
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