. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 102 S. Y. FENG separately with aluminum foil, dropped into liquid nitrogen for quick freezing and -lured iu the refrigerator at '.. to be sectioned with a freezing microtome. The sections placed on glass slide> were fixed briefly with lO^o formalin in sea water. They were then dehydrated in three changes of dioxane of four minutes each and mounted in non-fluorescent medium. The distribution of fluorescent proteins in tissues was ascertained by noting (1) the presence or absence of inocu- lated substances free or pinocytos
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 102 S. Y. FENG separately with aluminum foil, dropped into liquid nitrogen for quick freezing and -lured iu the refrigerator at '.. to be sectioned with a freezing microtome. The sections placed on glass slide> were fixed briefly with lO^o formalin in sea water. They were then dehydrated in three changes of dioxane of four minutes each and mounted in non-fluorescent medium. The distribution of fluorescent proteins in tissues was ascertained by noting (1) the presence or absence of inocu- lated substances free or pinocytosed in selected lumina (blood vessels, intestine, digestive diverticulum. etc. ), and (2) migration of protein-laden leucocytes through various epithelia. ioo 80" 60- " 40- o .1 20- Q_ Effect of temperature 5xlO~lgm%. 24° C. 10° C. 0 10 20 30 40 50 Minutes FicukK 3. The effect ot' temperature and time of exposure on the pinocytosis of rhodamine- labelled Inmian gamma globulin by oyster leucocytes. Each point on the graph represents the median of three samples. Regardless of the fact that the inocula consisted of three different protein so- lutions. no outstanding differences were found in their distribution or in the subse- quent elimination of the inoculated materials from the oyster. Thus, unless other- wise noted, the following results apply to all three inocula. The blood in the arterial and venous systems (anterior aorta, posterior aorta leading to the adductor muscle, hlood spaces in the muscle, small arteries in the visceral mass, blood spaces immediately adjacent to the stomach, intestine, style sac and rectum, sinuses in the mantle, medial gill axis vein and lateral palliobranchial veins) appeared to be filled with the labelled proteins 15 minutes after intracardial injection. At no time during the experiment were labelled protein^ detected in the circumpallial Please note that these images are extracted from scanned page images that may have been
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
