. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Chapter XIII 139 — Vital Staining. We have tried, by cultures of various plants in nutrient media to which neutral red has been added, to follow under the micro- scope the development of the vacuoles during cellular growth. This has, in addition, made it possible for us to judge the degree of toxicity of this dye. We have been able to obtain cultures of Sapro- legnia, for example, on soy bean bouillon to which of neutral red or neutral violet have been added. The cultures were grown in Petri dishes (Fig. 88)
. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Chapter XIII 139 — Vital Staining. We have tried, by cultures of various plants in nutrient media to which neutral red has been added, to follow under the micro- scope the development of the vacuoles during cellular growth. This has, in addition, made it possible for us to judge the degree of toxicity of this dye. We have been able to obtain cultures of Sapro- legnia, for example, on soy bean bouillon to which of neutral red or neutral violet have been added. The cultures were grown in Petri dishes (Fig. 88) whose bottoms had a 3 cm. opening covered by a cover slip sealed by asphalt cement (model used at the International Bureau for the Culture of Fungi at Baarn). It suf- ficed to turn the box over and place it under the microscope to be able to follow the devel- opment of the fungus under the oil immer- sion lens and to follow the life history of the vacuoles. Various species of Saprolegnia cul- tivated undere these conditions developed as well as those in the control cultures and fol- lowed out their entire life history from ger- mination of the zoospores to the formation of zoosporangia. Now, during all their develop- ment the dye accumulated in their vacuoles and stained them superbly. In solutions of neutral red beginning with a concentration of , growth is somewhat retarded. The fungus can stand relatively large doses of neutral red (), although when the concentration of the dye is above a cer- tain paint, it grows only very little. With DuFRENOY and Labrousse, we were able to germinate seeds of tobacco in pure culture on Knop's solution to which had been added doses of neutral red from We succeeded in doing the same with grains of wheat, barley and lupin seeds, in collaboration either with Obaton or with Gautheret. The seeds germinated normally under these conditions and by examining their roots in the Petri dish under a microscope it was po
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