. Electron microscopy; proceedings of the Stockholm Conference, September, 1956. Electron microscopy. Structural Details of Natural Fibers as Observed with the Electron Microscope C. Maertens, G. Raes, and G. Vandermeerssche Laboiatoire de Technologie des Matieres Textiles de /'Uiiiversite de Gaiid and Centre de Microscopie Electionique, Medical, Industrie! et Agricole. Most studies with the electron microscope on the fine structure of natural fibers have been limited to the suspension and to the replica techniques. This is mainly due to the fact that cellulose fibers are ver
. Electron microscopy; proceedings of the Stockholm Conference, September, 1956. Electron microscopy. Structural Details of Natural Fibers as Observed with the Electron Microscope C. Maertens, G. Raes, and G. Vandermeerssche Laboiatoire de Technologie des Matieres Textiles de /'Uiiiversite de Gaiid and Centre de Microscopie Electionique, Medical, Industrie! et Agricole. Most studies with the electron microscope on the fine structure of natural fibers have been limited to the suspension and to the replica techniques. This is mainly due to the fact that cellulose fibers are very hard to cut with the usual glass knives and that no quite appropriate staining techniques for cellulose have been found. At the Conference of Barcelona in 1954 (4) and at the Conference of Brussels in 1955 (2) we have mentioned already our first attempts of making ultra-thin sections of cotton fibers. Several staining methods had been tried and three of those had given good results: osmic acid, ferri- alum and iodine zinc chloride. A still better specific electron staining has been found by Saara Asunmaa (1) who has made ultra- thin sections of cellulose fibers after treatment with thallous ethylate. The contrast obtained by this method is excellent but unfortunately the method of staining is quite long and elaborate. We have been able to obtain equally well stained sections of cellulose fibers by a very simple treatment with silver nitrate. The fibers under investigation were dipped into a 20 % silver nitrate solution for a couple of hours and then washed, dehydrated and embedded in the usual way as described in our previous work (2). A mixture of 6/4 butyl-methyl-methacrylate has been found most suitable. The microtome used was the Porter Blum model and the sections obtained were on the average about 250 A thick. The electron microscope used was the Philips type EM 75. Cotton fibers of different types and different origin * k«>.*#»,. â â â » have been tried.
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