. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. Well-distributed colonies in agar inportion of Petri dish. Wolfifhiigels apparatus for counting colonies. best to make an emulsion of a portion of it, and use this rather thanthe original substance for making the dilutions to be used. Meas-ured quantities of the diluted material can be transferred most accu-rately through a sterihzed, long, glass pipette graduated in one one-hundredth cubic centimetres, or, more roughly, by a platinum loop ofknown size. Streaked Surfa


. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. Well-distributed colonies in agar inportion of Petri dish. Wolfifhiigels apparatus for counting colonies. best to make an emulsion of a portion of it, and use this rather thanthe original substance for making the dilutions to be used. Meas-ured quantities of the diluted material can be transferred most accu-rately through a sterihzed, long, glass pipette graduated in one one-hundredth cubic centimetres, or, more roughly, by a platinum loop ofknown size. Streaked Surface Plate Cultures.—About 8 of agar-agar arepoured into a Petri dish and allowed to harden. The substance tobe tested bacteriologically, or a dilution of it, is then drawn lightlyacross the surface of the medium in a series of parallel streaks bymeans of a platinum loop. Each successive streak is made with thesame needle or loop without replenishing the material to be streak will therefore leave less deposit of bacteria and fewercolonies will develop. While in the former method (poured plate)most of the


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Keywords: ., bookcentury1900, bookdecade1910, bookpublishernewyo, bookyear1910