. Bulletin. Insects; Insect pests; Entomology; Insects; Insect pests; Entomology. EGG PARASITES OF THE GIPSY MOTH. 171 Fig. 13.—Anastattis bifas- ciatus: Uterine egg. Greatly "enlarged. (Original.) tain in the hope that laboratory reproduction could be secured was soon recognized to be a mistake, and as the Anastatus continued to emerge considerably ahead of the time when they would obviously have issued under more natural conditions, it was resolved to remedy the evil, if possible, by placing the parasitized material in cold stor- age. This experiment was successful. The further transfor


. Bulletin. Insects; Insect pests; Entomology; Insects; Insect pests; Entomology. EGG PARASITES OF THE GIPSY MOTH. 171 Fig. 13.—Anastattis bifas- ciatus: Uterine egg. Greatly "enlarged. (Original.) tain in the hope that laboratory reproduction could be secured was soon recognized to be a mistake, and as the Anastatus continued to emerge considerably ahead of the time when they would obviously have issued under more natural conditions, it was resolved to remedy the evil, if possible, by placing the parasitized material in cold stor- age. This experiment was successful. The further transformations of the parasites were retarded without any apparent prejudicial effects upon their vitality, and in July some 500 were reared and colonized in the field. Coincidently with the height of their emergence and subsequent to its close, a considerable number of a small black encyrtid, later described by the senior author as Tyndarichus navx, issued, and all were destroyed on the supposition that they might be secondary. This was not by any means certain, and it was resolved to investigate their habits thoroughly so soon as opportunity should offer. Accordingly, in the fall of 1908, following the receipt of several con- siderable shipments of egg masses from Japan, an exhaustive investi- gation of the gipsy-moth egg para- sites was inaugurated. These in- vestigations were more intimately associated with the work upon Schedius, and more will be said of them in the discussion of that species. So far as Anastatus was concerned, its life and probable habits stood revealed from the start. Almost in the beginning its larvae were found (fig. 14) and identified correctly, as was later proved. They were almost invariably found in eggs which had been destroyed before embryonic development had taken place, which showed conclusively that these eggs were attacked within a very short time after their dep- osition. It was known that the adults did not issue until after the caterpillars had


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