. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. DISTRIBUTION OF SUBSTANCES IN SPERMATOZOON. 247 3. SCHUMACHER'S METHODS. A. One makes a smear with sperm as thick as possible, and before the sperm is dried the slide is plunged into HC1 solution I : 4. The slides remain in the acid 24 hours. After that time, wash in distilled water and stain for 5 minutes in I per cent. Victoria blue in distilled water. Again 5 minutes in distilled water. Mount after usual treatment in cedar oil, or, in order to have clearer pictures (but more unstable) one can mount directly in water and s
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. DISTRIBUTION OF SUBSTANCES IN SPERMATOZOON. 247 3. SCHUMACHER'S METHODS. A. One makes a smear with sperm as thick as possible, and before the sperm is dried the slide is plunged into HC1 solution I : 4. The slides remain in the acid 24 hours. After that time, wash in distilled water and stain for 5 minutes in I per cent. Victoria blue in distilled water. Again 5 minutes in distilled water. Mount after usual treatment in cedar oil, or, in order to have clearer pictures (but more unstable) one can mount directly in water and seal with paraffin. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
