. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 86 J. W. LYNN ET '/* • ' • ;*/.*?tf *•'• "*j?* ' %-*.i*;:j • v^iii^ft§?ii . Figure 1. A phase micrograph of a Trachypenaeus \imiln egg spawned directly into seawater containing fixative. Note birefringence of the cortical region of the egg (arrowhead) and the irregular egg shape. Bar = 100 Mm. Figure 2. The vitelline envelope overlying the egg plasma membrane and cortical crypts of T. similis are apparent in this SEM. Note the openings of the underlying crypts where the vitelline envelope has been disturbed (arrow)
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 86 J. W. LYNN ET '/* • ' • ;*/.*?tf *•'• "*j?* ' %-*.i*;:j • v^iii^ft§?ii . Figure 1. A phase micrograph of a Trachypenaeus \imiln egg spawned directly into seawater containing fixative. Note birefringence of the cortical region of the egg (arrowhead) and the irregular egg shape. Bar = 100 Mm. Figure 2. The vitelline envelope overlying the egg plasma membrane and cortical crypts of T. similis are apparent in this SEM. Note the openings of the underlying crypts where the vitelline envelope has been disturbed (arrow) and their non-uniform diameters. Bar = 50 ^m. Figure 3. The relationship between the thin vitelline envelope (arrowheads) and the underlying cortical crypts can be observed in light micrographs of thick plastic sections. Bar = 10 ^m. Figure 4. In this TEM of a T. ximilix egg crypt, the jelly precursor material appears as a diffuse libnllar material. The egg cytoplasm contains cisternae (arrows) with electron-dense material. The vitelline envelope is a thin, fluffy material overlying the crypts (arrowhead). Bar = 1 fim. Statistical analysis Comparisons of dimensional changes of total egg + hatching envelope diameter between control and ex- perimental samples were performed using Instat (Graph- pad Software, Inc.) for the Student's /-test using a two- tailed test with test for significance of P < Number of trials for each comparison are given as n. Results Morpholoi f. similis egg activation Although tin mrphology of the early activational events observed \vned eggs of Trachypenaeits similis is very similar 10 that described in Penaens sp. and Sicyonia in^fi, Clark et a/., 1980, 1990; Lynn el ai. 1991), a description is provided here for the hrst time to illustrate the similarities and the minor differences. Eggs of T. similis spawned into seawater and fixed within 1 min are approximately 220 ^m in diameter. A bright refractile ring in the cortex of the egg
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
