. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 76 MAX KRAUSS tale formed, depending upon the concentration of the solution. In Figure 1 a series of photographs of the mucin clot reaction of S. [>nr[>nr<itns fertilizin is shown, with the reaction given by hyaluronic acid for comparison. By taking advantage of the biological activity of fertilizin, , its sperm- agglutinating activity, a simple test was performed which provided definitive evi- dence that it is the fertilizin which is co-precipitated with serum albumin and not some hitherto undetected component
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 76 MAX KRAUSS tale formed, depending upon the concentration of the solution. In Figure 1 a series of photographs of the mucin clot reaction of S. [>nr[>nr<itns fertilizin is shown, with the reaction given by hyaluronic acid for comparison. By taking advantage of the biological activity of fertilizin, , its sperm- agglutinating activity, a simple test was performed which provided definitive evi- dence that it is the fertilizin which is co-precipitated with serum albumin and not some hitherto undetected component of the FIGURE 1. Mucin clot reactions of Strongylocentrotus pitrpitnttns fertilizin and of hy- aluronic acid in two-fold dilutions, a. Purified S. purfitratus fertilizin. The first tube contains fertilizin solution and serum albumin, but distilled water instead of acetic acid. The opacity of the first tube is due to the opalescence of the mixture, b. Crude S. purpuratus fertilizin. The first tube contains fertilizin solution and acetic acid but per cent saline instead of serum albumin, c. The same as b with the tubes shaken prior to being photographed, d. Hyaluronic acid. A solution of purified fertilizin in per cent saline was mixed with serum albumin and the pH brought to about with 2 N acetic acid. The resulting precipitate was thrown down by centrifugation, the supernatant withdrawn and its pH adjusted to 7. As shown in Table I, the supernatant exhibited no sperm- agglutinating Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. :
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
