. Calcified tissues; proceedings. Bone; Collagen; Calcification. |*>* ^. Fig. 4. Serial section to Fig. 3 showmi; ,i sent in the chondrocytes but has disappc GUcogen (arrow) is pre- ection in 90% cohol'tormalin. Non-specific esterase activity was present in the same distribution as acid phos- phatase and showed as small positive granules in the chondrocyte cytoplasm. Similar granules were seen in the acid phosphatase preparations. These observations indicate the presence of lysosomes in the chondrocytes which would be in accord with recent views on the mechanism of cartilage res
. Calcified tissues; proceedings. Bone; Collagen; Calcification. |*>* ^. Fig. 4. Serial section to Fig. 3 showmi; ,i sent in the chondrocytes but has disappc GUcogen (arrow) is pre- ection in 90% cohol'tormalin. Non-specific esterase activity was present in the same distribution as acid phos- phatase and showed as small positive granules in the chondrocyte cytoplasm. Similar granules were seen in the acid phosphatase preparations. These observations indicate the presence of lysosomes in the chondrocytes which would be in accord with recent views on the mechanism of cartilage resorbtion (Fell, 1964; Sledge and Dingle, 1965). On the other hand the chondrocytes in the mineralising zone are probably dying cells so it is possible that the acid hydrolase positive bodies could represent autophagic vacuoles (de Duve, 1963). Alkaline phosphatase appeared in mature chondrocytes just prior to mineralisa- tion and was stronger in chondrocytes within the mineralising zone. The highest levels of alkaline phosphatase were seen in osteoblasts and in periosteal cells of the condylar process. These findings confirm for this tissue the well documented associ- ation of alkaline phosphatase with cells involved in calcification (see Cabrini, 1961 for review).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original European Symposium on Calcified Tissues; European Bone and Tooth Symposium. Berlin, New York [etc. ] Springer-Verlag [etc. ]
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