American journal of physiology . rough which the juicewas drawn off, lower down. Collections fromthe container were made at one to two hourintervals depending on the rate of Acidity. — The free and total aciditieswere obtained by titration, using dimethyl-amino-azo-benzene and phenolphthalein as in-dicators. In the case of cats 2 to 14, i ofthe sodium hydroxide used was equivalent mg. of HCl; in the remaining cases was equivalent to ^g- of HCl. The latter strengthwas quite satisfactory since it required to cc. of thealkali to neutralize i of th
American journal of physiology . rough which the juicewas drawn off, lower down. Collections fromthe container were made at one to two hourintervals depending on the rate of Acidity. — The free and total aciditieswere obtained by titration, using dimethyl-amino-azo-benzene and phenolphthalein as in-dicators. In the case of cats 2 to 14, i ofthe sodium hydroxide used was equivalent mg. of HCl; in the remaining cases was equivalent to ^g- of HCl. The latter strengthwas quite satisfactory since it required to cc. of thealkali to neutralize i of the juice. 5. Digestive Activity. — Since the quantities of the juicesavailable were small and the number of determinations to bemade large, none of the more recent methods based on theaddition of varying concentrations of enzyme to a fixed quan-tity of substrate could be used. The method finally adoptedconsisted in taking the time required by i of juice to digest10 mg. of dry ox fibrin. The fibrin was washed, boiled, and. Figure 1. Device for collecting the juicefrom the Pawlowstomach pouch in catsExplanation in text. Secretion of the Gastric Juice during Parathyroid Tetany 29 preserved in alcohol. It was then shredded, cut into smallpieces of approximately the same size, and dried in a vacuumdesiccator. All the determinations were run on the same sampleof fibrin. The end point is necessarily an arbitary one for theindividual, for there are all variations in the size of the particlesbefore the complete solution of the fibrin. In juices which willdigest the 10 mg. in i to 3 hours the end point can be readwithin a range of 15 minutes; but for juices requiring 7 to 8hours the limit is more often 30 minutes. In many of thesamples mucus was present in large quantities, especially towardsthe end of the digestive period or during tetany. In such casesthe juice was centrifuged, if a sufficient sample was obtained;if not, a dilution with water was made and the mixture placedin a
Size: 1054px × 2371px
Photo credit: © The Reading Room / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookautho, bookcentury1800, bookdecade1890, booksubjectphysiology
