. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 126 F. CHEE AND M. BYRNE. Figure 2. Confocal optical projections of early hipinnariae. (A) Early bipinnaria reconstructed from 32 optical sections. Cells with 5-HT-like immunoreactivity can be clearly seen around the stomodaeal invagination (si) and the dorsal surface (arrowheads) of the larva. (B) Projection of 5 confocal sections from the ventral side of a 48-h bipinnaria. 5-HT-like immunoreactivity is present in cell bodies (arrowheads) in the adoral ciliated band (adcb), and a few immunoreactive cells are also present on
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 126 F. CHEE AND M. BYRNE. Figure 2. Confocal optical projections of early hipinnariae. (A) Early bipinnaria reconstructed from 32 optical sections. Cells with 5-HT-like immunoreactivity can be clearly seen around the stomodaeal invagination (si) and the dorsal surface (arrowheads) of the larva. (B) Projection of 5 confocal sections from the ventral side of a 48-h bipinnaria. 5-HT-like immunoreactivity is present in cell bodies (arrowheads) in the adoral ciliated band (adcb), and a few immunoreactive cells are also present on the upper left-hand side of the mouth (m). (C) Projection of 5 optical sections from the dorsal side of the larva in panel B. Immunoreactive cell bodies (neb) and axonal-like processes (ax) form a band partially wrapping around the larva. Bars. 95 incorporated into the serotonergic tracts associated with the preoral and postoral ciliated bands. As the preoral, postoral and adoral ciliated bands devel- oped, the oral hood was also beginning to form (Fig. 3A, B). Serotonin-like immunoreactivity was observed along the ciliated bands in the form of monopolar cell bodies with axonal-like tracts following the path of these bands (Fig. 3A). By this stage, a ganglion was evident at the anterior end of the larva. This anterior ganglion (AG) consisted of the immunopositive cells and processes innervating the preoral and postoral ciliated bands and processes intercon- necting these bands (Fig. 3A). Advanced bipinnaria (about 18 days old) underwent a distinct shape change with the formation of an extension at the anterior end of the larva (Fig. 4A, B). The three ciliated bands were well developed in these larvae (Fig. 4A, B). Internally, the larva had a well-developed gut. and the right and left enterocoels had formed. The distribution of immu- noreactive cells in these bands, discussed below, was con- sistent in all larvae examined (n = 100). Adoral cilititeil huiul The adoral cili
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