. Pharmaceutical bacteriology. Bacteriology; Disinfection and disinfectants. 7° PHARMACEUTICAL BACTERIOLOGY The essential requirements given under a, b, and c are obtained by filtration, neutralization, and sterilization, as will be more fully explained. Non-liquefiable solid media, as potato, bread, squash, etc., must be clean, free from living microbes and other organisms, and there should be a comparatively smooth ejqposed inoculating surface. These requirements are attained by washing and otherwise cleansing, disinfecting, rinsing, and heat sterilization (dry heat, steam or hot-water bath)
. Pharmaceutical bacteriology. Bacteriology; Disinfection and disinfectants. 7° PHARMACEUTICAL BACTERIOLOGY The essential requirements given under a, b, and c are obtained by filtration, neutralization, and sterilization, as will be more fully explained. Non-liquefiable solid media, as potato, bread, squash, etc., must be clean, free from living microbes and other organisms, and there should be a comparatively smooth ejqposed inoculating surface. These requirements are attained by washing and otherwise cleansing, disinfecting, rinsing, and heat sterilization (dry heat, steam or hot-water bath).. Pig. i8.—Arnold Steam and Hot-Air Sterilizer for Surgical Instruments. This sterilizer is a combination and portable sterilizer, so designed that instruments may be both sterilized and then dried by hot air, if desired. About ioo° C. can be attained with the hot air by simply turning the valve shown in the illustration, which turns the steam as it escapes from the chamber into the base. The following are the more important media: A. Nutrient Bouillon.— Beef Extract (Armtfur's, Liebig's, etc.), 3 gm Peptone, lo gm Salt, S gm Distaied Water, lOOO CO. Mix ingredients and boil for a few minutes. Filter through filter paper. This bouillon may be modified by adding glycerin (6 per cent.), and sugars, as dextrose, saccharose, or lactose (i per cent.). B. Loeffler's Blood Serum.—Very largely used in making diagnostic diphtheria bacillus cultures. In many cities this medimn, with sterilized cotton swabs, in sterilized test-tubes, is furnished free to physicians by the board of health. In cities and towns where this is not done, the pharmacist should be prepared to furnish the materials to the physicians. The medium consists of — Bouillon with i per cent. Glucose, Blood Serum, I part. 3 parts. The bouillon is prepared as above described, with i per cent, of glucose added. The blood serum can be obtained from calf, sheep, ox, or cow, through the butcher or at the abattoir. Co
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