. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. 8 254 Phylum Arthropoda molted from nymphs may be stored a year or more without excessive mortality by placing them in pyrex cylinders or "longevity ; These tubes are 8" in length by i%" in diameter. One end is plugged with well packed earth or with Plaster of Paris for a distance of 2", and a few coarse wood shavings or dried leaves are added so that the ticks are not forced into direct contact with the plug (Fig. 56). From 300
. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. 8 254 Phylum Arthropoda molted from nymphs may be stored a year or more without excessive mortality by placing them in pyrex cylinders or "longevity ; These tubes are 8" in length by i%" in diameter. One end is plugged with well packed earth or with Plaster of Paris for a distance of 2", and a few coarse wood shavings or dried leaves are added so that the ticks are not forced into direct contact with the plug (Fig. 56). From 300 to 400 ticks are placed in the tube and the latter is closed by a piece of muslin tied over the open end. The tubes are placed upright inside 4" galvanized metal cylinders set in the ground to their full length. The plugged end of the pyrex cylinder must be in close contact with moist soil. Moisture is supplied to the surrounding soil as necessary. The ticks are thus free to seek favorable humidity conditions. The "tick yard" in which the tubes are placed must be constructed or located so that the direct sun rays will not fall on the tubes. During the winter the tubes may either be left in the ground or stored indoors at 6° C. The ticks will survive outdoor conditions unless subjected to unusually abrupt transitions of temperature, particularly from cold to heat. For periods of at least 6 months adult ticks may be stored in pill boxes at 6° C. with the relative humidity maintained at about 80%. Engorged female ticks may be stored to delay oviposition for periods up to 4 months in card- board pill boxes at temperature and humidity conditions as stated above. Immature Stages. Unfed larvae may be stored for periods up to 2 months under the conditions just stated. Unfed nymphs may be kept successfully for periods up to 6 months in longevity tubes in the tick yard in the manner described for adult ticks. However, equally satisfactory results will ordinarily be obtai
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