. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. Small incubator. Thermoregulator. strong glass tube or metal case, to make use of the body heat by puttingit under the clothing next to the body or sleeping upon it. Naturally,this should only be done when other means fail. Methods for Obtaining Anaerobic Conditions for Bacteria.—Pasteur excluded the oxygen by pouring a layer of oil on the culturefluid. A simple device is that of Koch, who placed a thin strip ofsterile mica upon the agar or gelatin while still fluid i
. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. Small incubator. Thermoregulator. strong glass tube or metal case, to make use of the body heat by puttingit under the clothing next to the body or sleeping upon it. Naturally,this should only be done when other means fail. Methods for Obtaining Anaerobic Conditions for Bacteria.—Pasteur excluded the oxygen by pouring a layer of oil on the culturefluid. A simple device is that of Koch, who placed a thin strip ofsterile mica upon the agar or gelatin while still fluid in the Petri dish,after inoculation. After the solidification of the media the portionunder the mica is excluded from the air and anaerobic growth candevelop. A second simple method (Liborius) is to fill the tubes with mediafuller than usual and to inoculate the bacteria deep down to nearthe bottom of the tubes while the media are still semisolid. Ananaerobic growth will take place in the lower part of the tube. Ina similar way the closed arm of the fermentation tube will suffice foranaerobic growth, if the opening conne
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