. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 222 The Cultivation of Anaerobic Organisms floats upon the surface of the medium, melts during sterilization, but does not mix with it, and "sets" when cool. The inoculation is to be made while the culture medium is warm, after boiling and before the paraffin sets. Koch studied the colonies of anaerobic organisms by cultivating them upon a film of gelatin covered by a thin sheet of sterilized mica, by which the air was excluded. Salamonsen has
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 222 The Cultivation of Anaerobic Organisms floats upon the surface of the medium, melts during sterilization, but does not mix with it, and "sets" when cool. The inoculation is to be made while the culture medium is warm, after boiling and before the paraffin sets. Koch studied the colonies of anaerobic organisms by cultivating them upon a film of gelatin covered by a thin sheet of sterilized mica, by which the air was excluded. Salamonsen has made use of a pipet for making anaerobic cultures. It is made of a glass tube a few miUimeters in diameter, drawn out to a point at each end. The inoculated gelatin or agar-agar is drawn out to a point at each end. The inoculated gelatin or agar-agar is drawn in while hquefied and the ends sealed. The tube, of course, contains no air, and perfect anafirobiosis results. Theobald Smith has found the fermen- tation-tube and various modifications of it excellently well adapted to the growth of anaerobes, which, of course, grow only in the closed limb. Hens' eggs have been used for anaerobic cultures, and in them the tetanus bacillus grows remarkably well. Conditions of anaerobiosis are, however, not perfect, as can be shown by the behavior of the egg itself. If oxygen be completely shut out by oihng or varnishing the shell, a fertile egg will not develop. A quite satisfactory and simple device for routine work with anaerobic organisms has been invented by Wright.* The es- sential feature consists of a pipet, D, with a rubber tube, E, at the end, and one in- terruption connected by a rubber tube, C. The device wiU be made clear at once by a glance at the accompanying illustration. The method of employment is very simple. An ordinary tube of bouillon or other fluid the pipet, the whole being sterilized, the The bouillon being inoculated with the. 62, 63.—Wriglit's method of making
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