. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 84 K KOIKK ET AL. 1 . -\ v /•• rw^HBk iwwH111 F\\^lU^Sm ASBh*..'- , iOOPSfff * TFVsf^fe. * * Figure 4. Light micrographs from the video-captured images of microalgae after SLL-2 treatment and in control wells, (a) GymnnJiimim cutcminim forming cell chains (control), (b) G. cutenatum 24 h after SLL-2 (100 ; ml"1) treatment showing u swollen cell (an arrow) and debris from destroyed cells (arrowheads), (c) Actively swimming Prorocentnun micnn\ (control!, (d) P. inictin* 24 h after SLL-2 (100 /ig ml ') treatment showing
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 84 K KOIKK ET AL. 1 . -\ v /•• rw^HBk iwwH111 F\\^lU^Sm ASBh*..'- , iOOPSfff * TFVsf^fe. * * Figure 4. Light micrographs from the video-captured images of microalgae after SLL-2 treatment and in control wells, (a) GymnnJiimim cutcminim forming cell chains (control), (b) G. cutenatum 24 h after SLL-2 (100 ; ml"1) treatment showing u swollen cell (an arrow) and debris from destroyed cells (arrowheads), (c) Actively swimming Prorocentnun micnn\ (control!, (d) P. inictin* 24 h after SLL-2 (100 /ig ml ') treatment showing cytoplasm ejected from the theca (arrows) and numerous empty thecae (arrowheads), (e) Tetnmflinit. sp. cells are in rapid motion and are blurred on this image (control), (f) cells forming cell clumps without motility. plast large subunit (23S) rDNA sequence (DDBJ Accession No. AB159231) (19), was not available. For these reasons, our experimental model is somewhat indirect tor assessing the chemical recognition that actually occurs between the host and the algae. However, the facts that the responses of Symbiodinium to the lectin varied significantly among the strains used and that the clade of Symbuxliniitm in S. loch- nioilcs was uniform strongly suggest that some selection analogous to our experiment had taken place in the coral- algae system. Therefore, further experiments using an actual Symbiodinium isolate of the coral or a culture of clade C will be of interest. Of note, our results indicated that the action of SLL-2 toward Symbiodinium is mediated not only by carbohydrate- binding activity, but also by more specific but still uniden- tified mechanisms, since the peanut lectin that recognizes a o-galactosyl moiety such as SLL-2 was inert in our models. Our finding of lectin-mediated chemical recognition and physiological modulation between symbiotic dinoflagellates and a coral is a novel one. Identifying the protein respon- sible for this process can i
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
