. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PH -3 -1 log[cr FIGURE 3 (left). pH optimum. Open circles: egg amylase; solid circles: mammalian sali- vary amylase. FIGURE 4 (right). Effect of chloride on enzyme activity. In connection with a point to be discussed later, it is important to add that egg glycogen does not seem to be associated to particles sedimeriting as fraction I. In our working conditions, less than 10/£ of the total amount of glycogen could be detected in that fraction. Amylase activity The homogenates of B. arenarum eggs degrade glycogen in the same c
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PH -3 -1 log[cr FIGURE 3 (left). pH optimum. Open circles: egg amylase; solid circles: mammalian sali- vary amylase. FIGURE 4 (right). Effect of chloride on enzyme activity. In connection with a point to be discussed later, it is important to add that egg glycogen does not seem to be associated to particles sedimeriting as fraction I. In our working conditions, less than 10/£ of the total amount of glycogen could be detected in that fraction. Amylase activity The homogenates of B. arenarum eggs degrade glycogen in the same conditions as a-amylase. Thus, the pH of maximum activity is about and it decreases sharply out of the range (Fig. 3). The enzymatic activity is enhanced by chloride ions with an optimum concentration between and M (Fig. 4). Zir+ is inhibitory and 10^5 M HgCU showed no effect on the enzymatic activity (Table II). A Km value of was found. TABLE II Effects of ZnCl-2 and HgCli on amylase activity Additions Final concentration (M) Enzyme activity* Fraction I Salivary amylase ZnCU ID"3 10~2 87 70 79 74 10-1 0 8 HgCl2 10~5 98 — Activity without salts taken as Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
