. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. 202 BAOTERIOLOCIV from similar micrococci which occur in the methra by their being invariably decolorised by Gram's method, although otherwise they take the aniline stains very readily. If it is wished to demonstrate the gonooocoi in fresh gonorrhoeal secretion, the following is the best mode of procedure :âA drop of the pus is placed upon a cover-glass and spread out lightly, so that the glass is covered with a thin cloudy film; but the common method of laying two cover-glasse
. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. 202 BAOTERIOLOCIV from similar micrococci which occur in the methra by their being invariably decolorised by Gram's method, although otherwise they take the aniline stains very readily. If it is wished to demonstrate the gonooocoi in fresh gonorrhoeal secretion, the following is the best mode of procedure :âA drop of the pus is placed upon a cover-glass and spread out lightly, so that the glass is covered with a thin cloudy film; but the common method of laying two cover-glasses one over the other and then drawing them asunder must not be employed here. The cover-glass is â¢Â» » /Sife ;â '⢠â¢!⢠now stained with a con- centrated alcoholic solu- tion of methyl blue, rinsed in water, dried, and mounted in Canada balsam. Neisser has recom- mended the following method of double stain- ing :âThe cover - glass preparations are brought for some minutes into a warm concentrated alcoholic solution of eosine, the superfluous dye is then soaked up with blottmg-paper, the preparations laid for a quarter of a minute in concentrated alcoholic solution of methyl blue, and rinsed in water. Schiitz's method consists in preparmg a filtered satu- rated solution of methyl blue in 5 per cent, aqueous carbolic acid, in which the preparations are stained for five to ten minutes in the cold. After rinsing in water they are laid for an instant in very dilute acetic acid, again rinsed in water, and double-stained in very dilute solution of safranine. The gonococci are blue and the pus-cells Fig, 78.âGonococci from the Urethral Secretion. (After Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Schenk, Samuel Leopold, 1840-1902. London, Longmans, Green
Size: 1615px × 1547px
Photo credit: © The Book Worm / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1800, bookdecade1890, booksubjectbacteri, bookyear1893
