. Biological structure and function; proceedings. Biochemistry; Cytology. LIGHT-INDUCED PHOSPHORYLATION 439 which gives an appreciable degree of stimulation only in pre-aged prepara- tions [6]. However, also with PMS, in preparations with activities of about 600 /xmoles of orthophosphate esteriiied per hour per mg. chlorophyll, or chlorophyll (reduced) DPNH Succinate. cylochrofne c, (oxidized) Qscorbate ' Fig. 7. higher, the degree of stimulation has been relatively low, as low as 50% or less. The two agents must stimulate phosphate esterification bv bridging different parts of the electron tr
. Biological structure and function; proceedings. Biochemistry; Cytology. LIGHT-INDUCED PHOSPHORYLATION 439 which gives an appreciable degree of stimulation only in pre-aged prepara- tions [6]. However, also with PMS, in preparations with activities of about 600 /xmoles of orthophosphate esteriiied per hour per mg. chlorophyll, or chlorophyll (reduced) DPNH Succinate. cylochrofne c, (oxidized) Qscorbate ' Fig. 7. higher, the degree of stimulation has been relatively low, as low as 50% or less. The two agents must stimulate phosphate esterification bv bridging different parts of the electron transport chain, as antimycin A exerts a TABLE IV Menadione and PMS as Electron Carriers in LIP in Chromatophores of R. rubrum The experimental details were as described in ref. [3]. The final concentrations of the various agents were, where added: 3'3 x lo"^* m ATP, 10"^^ M menadione, and 3 3 X io~* M PMS. The time for the experiment was 20 min. in the absence and 6 min. in the presence of PMS. A /Ltmoles orthophosphate esterified//;/" ODgoo" Antimycin I II* No addition Menadione PMS No addition Menadione PMS o-i 25 28 91 7 13 1-5 i-i 76 0-4 0-3 74 66 * In experiment II the chromatophores had been partly destroyed by heating 10 min. in a water-bath of 55-57 immediately before the actual experiment. Strong inhibition only on the stimulation due to addition of menadione. This is seen especially clearly in the experiment with the pre-aged sample. A comparison between the values obtained with the fresh and the pre-aged material further show^s that the stability of the three systems increases in the order: system with no added electron carrier, system with added menadione, and system with added PMS. This sequence has been shown. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS Internati
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