. A laboratory manual of soil bacteriology. Soil microbiology. 68 SOIL BACTERIOLOGY 3. Select a large and firm nodule, cut off, and immerse for three to five minutes in mercuric chlorid solution (i : 500), and finally in alcohol. 4. Remove alcohol by flaming and place the nodule on a sterile surface (flamed slide). **. Fig. 5.—Bacillus radicicola from a root nodule of alfalfa; X 1200. 5. Cut open with a sterile knife and take out some of the inner contents. ^^ -i 6. Inoculate this bacterial mass from the nodule into a few drops of water in a Petri dish. 7. Make two or more loop transfers from
. A laboratory manual of soil bacteriology. Soil microbiology. 68 SOIL BACTERIOLOGY 3. Select a large and firm nodule, cut off, and immerse for three to five minutes in mercuric chlorid solution (i : 500), and finally in alcohol. 4. Remove alcohol by flaming and place the nodule on a sterile surface (flamed slide). **. Fig. 5.—Bacillus radicicola from a root nodule of alfalfa; X 1200. 5. Cut open with a sterile knife and take out some of the inner contents. ^^ -i 6. Inoculate this bacterial mass from the nodule into a few drops of water in a Petri dish. 7. Make two or more loop transfers from the first Petri. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Fred, Edwin Broun, 1887-1981. Philadelphia, London, W. B. Saunders Company
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