. The development of a cosmid map of chromosome 12p13. Sweet Briar College; Chromosomes. ^0*N^ 6,108 ââ â^^\^ 5,090 ^^â '->/^ 4,072. 396 344 298 220 201 154 134 a) b) " Figure 11. a) Electrophoresis of agarose gel. b) 1 KB ladder. This is the DNA of known fragment size that is run as a standard for size migration on all agarose gels that are electrophoresed. Detection of the bands by ultraviolet light requires the gel to either be made with ethidium bromide ( |ag/ml) or to be stained with ethidium bromide (~200ng/ml) before photographing (FIGURE 12). As ethidium bromide is a carcino
. The development of a cosmid map of chromosome 12p13. Sweet Briar College; Chromosomes. ^0*N^ 6,108 ââ â^^\^ 5,090 ^^â '->/^ 4,072. 396 344 298 220 201 154 134 a) b) " Figure 11. a) Electrophoresis of agarose gel. b) 1 KB ladder. This is the DNA of known fragment size that is run as a standard for size migration on all agarose gels that are electrophoresed. Detection of the bands by ultraviolet light requires the gel to either be made with ethidium bromide ( |ag/ml) or to be stained with ethidium bromide (~200ng/ml) before photographing (FIGURE 12). As ethidium bromide is a carcinogen, its use requires extreme caution and gels and buffers containing the solution must be properly destained before ;"" Another possible stain to use is SYBR Gold Nucleic Acid Stain, a proprietary unsymmetrical cyanine dye. This stain is one of the most sensitive available for detecting double or single-stranded DNA or RNA in electrophoretic gels. It is more sensitive than ethidium bromide in many applications and does not require destaining prior to visualization. In addition the dye can be readily removed from the nucleic acids leaving the templates pure for fixrther study.'^^ 22. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Belk, Stephanie Heather.
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Keywords: ., bookcentury1900, booksubjectchromo, booksubjectsweetbriarcollege
