. Animal micrology; practical exercises in microscopical methods. Microscopy -- Technique; Microscopes -- Technique. FIG. 38.—Cornet's Cover-Class Forceps. 2. Kinse in water. 3. Gram's solution (memorandum 3/) until the color becomes black (2 to 3 minutes). 4. Ninety-five per cent, alcohol until the violet color has almost com- pletely disappeared. 5. Rinse in water and examine by placing the cover-glass film side downward on a slide. Only a thin film of water should remain between the slide and the cover. Remove surplus water by means of blotting paper. If a prolonged examination is to be mad
. Animal micrology; practical exercises in microscopical methods. Microscopy -- Technique; Microscopes -- Technique. FIG. 38.—Cornet's Cover-Class Forceps. 2. Kinse in water. 3. Gram's solution (memorandum 3/) until the color becomes black (2 to 3 minutes). 4. Ninety-five per cent, alcohol until the violet color has almost com- pletely disappeared. 5. Rinse in water and examine by placing the cover-glass film side downward on a slide. Only a thin film of water should remain between the slide and the cover. Remove surplus water by means of blotting paper. If a prolonged examination is to be made, water lost by evapora- tion must be replaced by occasionally placing a small drop of water at. FIG. 39.—Stewart's Steel Wire Cover-Glass Forceps. the edge of the cover. In ordinary work the final inspection is frequently made at this stage. If a permanent preparation is desired, however, proceed with the following steps: 6. If the bacteria are well stained, a counterstain of Bismarck brown (memorandum 3d, sol. 2) may be added (5 to 10 seconds). This step may be omitted. 7. Absolute alcohol, 10 to 15 seconds. 8. Xylol. 9. Xylol-balsam. NOTE.—In staining, if the cover-glass is warmed over a (lame some 15 or 20 seconds until the stain steams, the action of the stain is usually more intense and more rapid. Boiling, however, must be Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Guyer, Michael F. (Michael Frederic), b. 1874. Chicago, The University of Chicago Press
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