. Pathological technique; a practical manual for workers in pathological histology and bacteriology. Fig. 83.—Bacillus of anthrax from spleen of a mouse (L. Frothingham). and show square or slightly concave ends. The capsule isnot present in cultures. PATHOGENIC BACTERIA AND FUNGI. 329 Stained by Grams method. Not motile. Forms oval spores in the middle of the short segments or » v £x si I •** * « m .J Fig. 84—Bacillus of anthrax, stained to show the spores; X 1000 (Frankel and Pfeiffer). rods. The spores may be seen in blood-serum cultures afterforty-eight hours in the incubator (Fig. 84).. F
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. Fig. 83.—Bacillus of anthrax from spleen of a mouse (L. Frothingham). and show square or slightly concave ends. The capsule isnot present in cultures. PATHOGENIC BACTERIA AND FUNGI. 329 Stained by Grams method. Not motile. Forms oval spores in the middle of the short segments or » v £x si I •** * « m .J Fig. 84—Bacillus of anthrax, stained to show the spores; X 1000 (Frankel and Pfeiffer). rods. The spores may be seen in blood-serum cultures afterforty-eight hours in the incubator (Fig. 84).. Fig. 85.—Bacillus of anthrax : gelatin stab-culture seven days old (Gunther). Blood-serum.—Irregularly rounded colonies, several milli-meters in diameter after twenty-four hours in the colonies are grayish, finely granular, and have the ap- 330 PATHOLOGICAL TECHNIQUE. pearance of being made up of a dense network of delicatefibrillar. The blood-serum is slowly liquefied. Gelatin Stab.—Growth along the line of stab, with radiat-ing filaments extending laterally into the gelatin, which isslowly liquefied in funnel form (Fig. 85). Bouillon.—Growth in the form of cotton-like flakes andfilamentous masses. No clouding of the medium. Agar-agar.—Matted network of translucent filaments. / / Fig. 86.—Bacillus of an-thrax; cover-slip prepara-tion from vesicle. Stainedby W. H. Smiths method ;X 1800 (W. H. Smith;photo, by L. S. Brown).
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