Clinical diagnosis : the bacteriological, chemical, and microscopical evidence of disease . nditions an excess of nitrogen may be demonstrated in theblood, and this is the expression of the urea present. V. Schroder has shown that the formation of urea probably takes place inthe liver. 4. Uric Acid and Xanthin Substances. 1. Uric Acid.—Garrod found uric acid to the amount of in a thousand in the blood of persons suffering from gout. Itmust be observed, however, that his method of testing for this substancewas far from He took about 30-35 grms. of blood and allowed it to c
Clinical diagnosis : the bacteriological, chemical, and microscopical evidence of disease . nditions an excess of nitrogen may be demonstrated in theblood, and this is the expression of the urea present. V. Schroder has shown that the formation of urea probably takes place inthe liver. 4. Uric Acid and Xanthin Substances. 1. Uric Acid.—Garrod found uric acid to the amount of in a thousand in the blood of persons suffering from gout. Itmust be observed, however, that his method of testing for this substancewas far from He took about 30-35 grms. of blood and allowed it to coagulate. Ten cc. ofthe serum were then mixed with a dilute acetic acid solution in the proportion of URIC ACID 85 1 : 10, and a delicate thread was placed in the fluid. When the blood containednot less than Per 1000 uric acid, it was found that, after twenty-four toforty-eight hours, the thread was covered with uric acid crystals. In a few instances only did he precipitate from the blood with alcohol andapply the murexide test, Abeles™ has freed the blood from proteids by the. Fig. Extraction-Apparatus as modified by i?. v. Jaksch. Schmidt-Mulheim process, and then tested for uric acid by Ludwig and SalJcowsUsmethod. Salomon*** has observed an increase of uric acid in the blood durino-the acute attack of gout. For the detection of uric acid in the blood, the following proceduremay be adopted :359—100-300 grms. of blood are removed by cupping,and at once diluted with 3-4 times the bulk of water, heated on the 86 THE BLOOD water-bath, and, when coagulation begins, treated with a few drops ofacetic acid (sp. gr. at 15° C.) until it has a feebly acid is left on the boiling water-bath for 15-20 minutes, then removedand filtered. The precipitate on the filter is repeatedly washed withhot water and added to the nitrate. The fluid, which should now havea slight yellow tinge, is again treated with a little acetic acid (sp. gr. asbefore), boi
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