. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. BACTERIOLOGICAL TECHNIC. 4, The essential requirements given under a, b, and c are obtained by filtra- tion, neutralization, and sterilization, as will be more fully explained. Non- liquefiable solid media, as potato, bread, squash, etc., must be clean, free from Uving microbes and other organisms, and there should be a compara- tively smooth exposed inoculating surface. These requirements are attained by washing and otherwise cleansing, disinfecting, rinsing, and heat sterili- zation (dry he
. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. BACTERIOLOGICAL TECHNIC. 4, The essential requirements given under a, b, and c are obtained by filtra- tion, neutralization, and sterilization, as will be more fully explained. Non- liquefiable solid media, as potato, bread, squash, etc., must be clean, free from Uving microbes and other organisms, and there should be a compara- tively smooth exposed inoculating surface. These requirements are attained by washing and otherwise cleansing, disinfecting, rinsing, and heat sterili- zation (dry heat, steam or hot-water bath). The following are the more important media: A. Nutrient Bouillon.— Beef Extract (Armour's, Liebig's, etc.), 3 gm- Peptone, 10 gm. Salt, S gm. Distilled Water, 1000 Mix ingredients and boil for a few minutes. Filter through filter paper. This bouillon may be modified by adding glycerin (6 per cent.) and sugars, as dextrose, saccharose, or lactoSe (i per cent.). B. Loeffler's Blood Serum.—Very largely used in making diagnostic diphtheria bacillus cultures. In many cities this medium, with sterilized cotton swabs, in sterilized test-tubes, is furnished free to physicians by the board of health. In cities and towns where this is not done, the pharmacist should be prepared to fur- nish the materials to the physicians. The medium con- sists of. Bouillon with i per cent. Glucose, Blood Serum, I part. 3 parts. Fig. 19.—Culture tube and swab tube used by physicians in the diagnosis of diph- theria. The swab tube should be long enough to have the entire length of swab inside, not projecting as shown in the fig- ure. (Williams.) The bouillon is prepared as above described, with i per cent, of glucose added. The blood serum can be obtained from calf, sheep, ox, or cow, through the butcher or at the abattoir. Collect the blood in a clean, sterile jar or flask, closed with cotton plug. Place on ice for twenty-four to forty-eight hours, during which ti
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