. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 66 W. R. ECKBERG AND M. E. PEROTTI. FIGURE 4. Lytechinuspictus egg 30 s after insemination in ASW. Note the beginning of fertilization envelope (FE) elevation. Also note that sperm are attached wherever the fertilization envelope has not yet begun to elevate. FIGURE 5. Lytechinus pictus egg 30 s after insemination in 30 quercetin. Many sperm are attached to the vitelline layer of the egg, but there is no evidence of egg activation. FIGURE 6. Surface of a quercetin-treated Lytechinus pictus egg. The surface shows typical
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 66 W. R. ECKBERG AND M. E. PEROTTI. FIGURE 4. Lytechinuspictus egg 30 s after insemination in ASW. Note the beginning of fertilization envelope (FE) elevation. Also note that sperm are attached wherever the fertilization envelope has not yet begun to elevate. FIGURE 5. Lytechinus pictus egg 30 s after insemination in 30 quercetin. Many sperm are attached to the vitelline layer of the egg, but there is no evidence of egg activation. FIGURE 6. Surface of a quercetin-treated Lytechinus pictus egg. The surface shows typical microvilli (MV) and the cortical granules (CG) are intact. Bar = Mm. FIGURE 7. Electron micrograph of a Lytechinus pictus sperm bound to the surface of an egg 30 s after insemination in 30 ^M quercetin. The acrosomal filament (AF) has attached to the vitelline layer (VL) but the acrosomal process is still surrounded by a continuous membrane. An intact cortical granule (CG) can be seen adjacent to the attached sperm. Bar = If vitelline layer penetration were prevented by quercetin, disruption of the vi- telline layer should restore fertilizability. However, trypsin disruption of the vitelline layer did not restore fertilizability (Table I). By 5 min after insemination, control eggs showed normal fertilization envelopes (Fig. 8), where quercetin-treated eggs still showed attached sperm but no evidence of activation (Figs. 9, 10). Subsequent treatment with ionophore A23187 resulted in fertilization envelope elevation and sperm detachment (Fig. 11). Electron mi-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfi
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