. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HHUIT -1. Homogenales of unactivated surf clam oocytes contain large, tandemly arranged, globular structures. In phase microscopy, a large, phase-dark sphere (large arrows) is associated with a small, round, phase-light particle (small arrows). These tandemly arranged particles are frequently associated with a phase- dark membrane-like structure (see particle on the left). A magnification bar with 10 ^m spacing is shown. in HGL and centrifuged at 2500g to pellet the paniculate tuhulin. An equal volume of each resuspended pel
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HHUIT -1. Homogenales of unactivated surf clam oocytes contain large, tandemly arranged, globular structures. In phase microscopy, a large, phase-dark sphere (large arrows) is associated with a small, round, phase-light particle (small arrows). These tandemly arranged particles are frequently associated with a phase- dark membrane-like structure (see particle on the left). A magnification bar with 10 ^m spacing is shown. in HGL and centrifuged at 2500g to pellet the paniculate tuhulin. An equal volume of each resuspended pellet was analyzed and compared by SDS-PAGE and immuno- blotting (Fig. 7B). Each 650g fraction—SI, S2, S3, and P—contained tubulin that sedimented at 2500g. The largest amount of paniculate tubulin was found in the fractions farthest away from the nucleoli (Table I). This does not rule out the possibility that a small amount of sedimentable tubulin still might be associated with the nucleoli. However, no nucleolar tubulin was detected by immunofluorescence with the DM 1A antibody. The lev- els of nucleolar fluorescence were indistinguishable from the controls in which no first antibody was added. To identify the source of the participate tubulin, a small sample of each resuspended pellet (2500g) was negatively stained with uranyl acetate for transmission electron mi- croscopy (Fig. 7D). The bottom three fractions contain numerous short microtubules which are associated with membranes and densely staining, amorphous aggregates. No microtubules are detected in the uppermost fraction (SI), which also contained numerous membranes, gran- ular aggregates, and the largest fraction of particulate tu- bulin. The abundance of membranes in all fractions suggested that the sedimentable tubulin might be membrane-bound. The amount of IPT that was detergent-soluble was ex- amined before and 5 min after parthenogenetic activation. Homogenates were prepared in HGL in the presence and absence
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
