. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. luciferase lactate / xx dehydrogenase/7. 371 DROPS - .05 P b \ a "c 5 o ZJ cr 0 ( ^o DAY EXTRACT N 100% RECOVERY ; o / \ 0 ^ 376 DROPS , s~\ TOT4L^- y ^^ ) 100 200 BOTTOM drops .05 FIGI/HK 1. Behavior of luciferase activity from a day and a night extract during sedi- mentation of the extracts through sucrose gradients, showing persistence of the difference be- tween day and night activities. Half of each of two 800 ml cultures was harvested 6 hours after lights on (DAY) and hours after lights off (NIGHT), extracted
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. luciferase lactate / xx dehydrogenase/7. 371 DROPS - .05 P b \ a "c 5 o ZJ cr 0 ( ^o DAY EXTRACT N 100% RECOVERY ; o / \ 0 ^ 376 DROPS , s~\ TOT4L^- y ^^ ) 100 200 BOTTOM drops .05 FIGI/HK 1. Behavior of luciferase activity from a day and a night extract during sedi- mentation of the extracts through sucrose gradients, showing persistence of the difference be- tween day and night activities. Half of each of two 800 ml cultures was harvested 6 hours after lights on (DAY) and hours after lights off (NIGHT), extracted in M tris, M EOT A, pH M DTT, centrifuged 40 min at X g, and stored at -57° C. Gradients were run as described under Methods. detaches, possibly covalently, to and from the "backbone" of the luciferase molecule during each circadian cycle. The presence of this moiety would alter the luciferase activity. Luciferase would thus occur in two forms, one more active than the other. In the absence of growth, the total number of luciferase "backbone" molecule? would remain constant. One of two opposite situations might obtain: (1) the moiety might cause a 10-fold change in activity ; every "day" luciferase molecule might then be 10% as active as every "night" molecule, in which case the active species would differ chemically from day to night; (2) the moiety might com- pletely activate or inactivate; all of the molecules would be active at night while only 10% of the molecules would be active by day. In this case the active species would be the same from day to Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lill
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
