. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 506 W. R. ELLINGTON AND A. C. MINES Table I \liiin honilru/l activities / phospliiiKen kinuses in the muscles oj a viinet i nl invertebrates. A "+" or "--" nulictilcs the presence or absence. rcs/ici 'lively, of mitochondria! kinase activity. Quality nl iniinchnmlrnil preparations is indicated by showing the range ul values i>l the respiratory control ratios (RCR = State 3 respiration -H State 4 respiration). An RCR value greater than one indicates thai mitochondria are coupled and sluw respiratory con
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 506 W. R. ELLINGTON AND A. C. MINES Table I \liiin honilru/l activities / phospliiiKen kinuses in the muscles oj a viinet i nl invertebrates. A "+" or "--" nulictilcs the presence or absence. rcs/ici 'lively, of mitochondria! kinase activity. Quality nl iniinchnmlrnil preparations is indicated by showing the range ul values i>l the respiratory control ratios (RCR = State 3 respiration -H State 4 respiration). An RCR value greater than one indicates thai mitochondria are coupled and sluw respiratory control behavior in response to the addition ol ADP (see discussion below) Organism and tissue Phosphagen Mitochondria] RCR kinase activity Lutnbricus terrestris body wall (earthworm) 3-4 LK Nereis virens body wall (polychaete) 4-5 GK Busycon cana/iculatuin radula muscle (whelk) 3-5 AK Octopus vulgaris systemic ventricle (octopus) 8-17 AK Sarcophaga bullaia flight muscle (blowfly) 4-7 AK Manduca se.\la flight muscle (moth) 5-10 AK Procambarus clarkii heart (crayfish) 4-6 AK + N. virens and B canalicitlatitin were obtained from the Marine Bio- logical Laboratory (Woods Hole. Massachusetts). L terrestris. S bullata. and M sextawete purchased from Carolina Biological Supply (Burling- ton, North Carolina). O vulgaris and P. clarkii were collected locally. Mitochondria were isolated by gentle homogenization and differential centnfugation procedures (details available upon request). Mitochondnal respiration was monitored polarigraphically as previously described (6. 7). The addition of ADP to tightly coupled mitochondria respiring in the presence of substrate (state-4) leads to a dramatic increase in respi- ration (state-3) which will continue until all of the ADP has been phos- phorylated to ATP. If a phosphagen kinase is present in the mitochondria, subsequent addition of the appropriate phosphagen acceptor (arginine, lombncine, glycocyamine, etc.) will lead to the formation of phosph
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
