. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. \. Figure 7. Light photomicrographs showing the spatial distribution ot imoplusm in control and com- pressed eight-celled embryos and in silu hybridizations of sectioned control and compressed cleavage- arrested eight-celled embryos using SpMA3C anti-sense RNA. (A) A detergent-extracted control embryo showing myoplasm contained in the blastomeres (long arrows). (B) Bright-field image of a cleavage- arrested embryo showing the hybridization of SpMA3C probe to the cytoplasm of blastomeres (long arrows). (C) A deterge
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. \. Figure 7. Light photomicrographs showing the spatial distribution ot imoplusm in control and com- pressed eight-celled embryos and in silu hybridizations of sectioned control and compressed cleavage- arrested eight-celled embryos using SpMA3C anti-sense RNA. (A) A detergent-extracted control embryo showing myoplasm contained in the blastomeres (long arrows). (B) Bright-field image of a cleavage- arrested embryo showing the hybridization of SpMA3C probe to the cytoplasm of blastomeres (long arrows). (C) A detergent-extracted, compressed embryo showing myoplasm in the blastomeres (long arrows) and in two additional blastomeres (short arrows). (D) Bright-field image of a compressed and cleavage-arrested eight-celled embryo showing the hybridization of SpM A3C probe to the cytoplasm in the blastomeres (long arrows) and in two additional blastomeres (short arrows). Scale bar in (A) equals 50 ^m. Same magnification for (B-D) as (A). were rinsed with PBS and then fixed in 2% glutaralde- hyde in M sodium phosphate buffer, pH , for 30 min at room temperature. Samples were washed three times in M sodium phosphate buffer. Fixed speci- mens were immersed in 1% osmium in the same buffer •or 1 h at room temperature, followed by dehydration rough a graded us of ethanol (10%, 30%, 50%, 70%, 100%) for 10 min at each step, nmens we ansferred into a specimen holder is inserted e a Tousimis Autosamdri-814 -loint drying hamber. Dried specimens were o double- d tape on an aluminum stub ' inside a Sputtering System, Hummer VII coati hine. A 20-nm gold/palladium metal alloy coating ipplied to the surface of each specimen. and the specimens were viewed using a JSM-6400 scanning electron microscope. In situ hybridization The in situ hybridization method previously described by Tomlinson et al. (1987) was used in the present study. Normal and compressed eight-celled embryos, cultured in s
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
