. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 434 MARIA DOLORES PEREZ-GONZALEZ The detergents employed and digitonin provoked only a partial release of hor- mone (Fig. 8). Part of the homogenate plus desoxycholate after one hour of in- cubation was heated and diluted in sea water, and greater activity was seen after this treatment. ///. Inactivation of the hormone In some experiments the homogenates of sinus glands in distilled water were injected into the whole crab (Uca pugilator). Figure 9 shows the degree and the UJ UJ a: o a_ o cr i o I. 10 20 30 40 50 TIME IN MINU
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 434 MARIA DOLORES PEREZ-GONZALEZ The detergents employed and digitonin provoked only a partial release of hor- mone (Fig. 8). Part of the homogenate plus desoxycholate after one hour of in- cubation was heated and diluted in sea water, and greater activity was seen after this treatment. ///. Inactivation of the hormone In some experiments the homogenates of sinus glands in distilled water were injected into the whole crab (Uca pugilator). Figure 9 shows the degree and the UJ UJ a: o a_ o cr i o I. 10 20 30 40 50 TIME IN MINUTES 60 FIGURE 7. Response of black chromatophores of Uca to injections of homogenates of sinus glands in isotonic sucrose before, +, and after heating at 70° C., O, and in boiling water, C; and after freezing and thawing, O. Homogenate in distilled water, • duration of the dispersion of chromatophores in relation to the concentration of the homogenates. The injection of ml. of a homogenate of sinus gland per ml., , the injection of an amount corresponding to sinus gland, is enough to cause a maximal dispersion of the chromatophores in almost 30 minutes and only four hours later have the chromatophores reached the stage of complete concentration again. It is interesting to notice that the time required for normal dark Uca to become pale after eyestalk removal is three to four hours. At all concentrations of homogenates dispersion was found to require less time than concentration of pig- ment within the chromatophores. The elimination of the hormone seems to be a very slow process. Even an injection corresponding to sinus gland (open. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). An
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