. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LIML'LIS AMEBOCYTE CYTOSKELETON 421. Figure 2B. A higher magnification of the marginal microtuhule hand (MB) in cross-section. Projections can be seen leading from one microtubule to another (». These projections may serve to stabilize the MB, and are likely related to microtubule-associated proteins present in other systems. • ization of the MB only occurred in endotoxin-activated cells. Nuclear profiles Amebocyte nuclei of cells studied under all three con- ditions (native, activated, and endotoxin-activated) had
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LIML'LIS AMEBOCYTE CYTOSKELETON 421. Figure 2B. A higher magnification of the marginal microtuhule hand (MB) in cross-section. Projections can be seen leading from one microtubule to another (». These projections may serve to stabilize the MB, and are likely related to microtubule-associated proteins present in other systems. • ization of the MB only occurred in endotoxin-activated cells. Nuclear profiles Amebocyte nuclei of cells studied under all three con- ditions (native, activated, and endotoxin-activated) had mean axial ratios of (native, n = 30), (activated, n = 29) and (endotoxin-activated, n = 30). These values fit the axial ratios for an oblate ellipsoid, which is the most common nuclear shape of discoid cells (Elias and Hyde, 1983). The slightly smaller mean axial ratios of the nuclei in the endotoxin-activated cells may be due to the change in cell shape from discoid to a more spheri- cal shape. These data were generated to determine if the nuclear profiles changed under the various conditions of the study, as we wished to know whether cytoskeletal changes were secondary to either an increase or decrease in nuclear size. Since nuclear axial ratios did not change appreciably under any of the experimental conditions, these data support our interpretation that the microtu- bule coil constricts around the nucleus in endotoxin-acti- vated amebocvtes. Discussion The discoid shape of native amebocytes has been ob- served with a variety of techniques, including high reso- lution differential interference contrast (DIG) micros- copy (Armstrong. 1979) and electron microscopy (Cope- land and Levin, 1985; Levin. 1985b). In this paper, we have presented data which suggest that the two major elements of the amebocyte cytoskeleton appear to act in tandem to maintain the discoid shape of the native amebocyte. The cortical actin array with its link to the plasma membrane, in
Size: 1877px × 1332px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
