. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PERMEABILITY OF TROUT ERYTHROCYTES 325 F(C,V) '- 0 i TIME IN MINUTES FIGURE 2. Experimental times plotted against F(C, V). Five additions of , , , and ml of M thiourea in NaCl were made to ml of cutthroat cells in NaCl. Only the last three additions were measured. Closed circles are for an intra- cellular concentration of and an extracellular concentration of X's are for an in- tracellular concentration of and an extracellula
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PERMEABILITY OF TROUT ERYTHROCYTES 325 F(C,V) '- 0 i TIME IN MINUTES FIGURE 2. Experimental times plotted against F(C, V). Five additions of , , , and ml of M thiourea in NaCl were made to ml of cutthroat cells in NaCl. Only the last three additions were measured. Closed circles are for an intra- cellular concentration of and an extracellular concentration of X's are for an in- tracellular concentration of and an extracellular concentration of Open circles are for an intracellular concentration of and an extracellular concentration of All concentrations are in isotones. with all four bloods and all four penetrants. In all 16 cases the experimental times when plotted against F(C, V) fell on a single straight line and in every case when these same times were plotted against F' (C,V) a family of straight lines was ob- tained. In Table I are summarized relative rates of penetration of the four non- electrolytes into erythrocytes of the four different species of trout. Graphs were made of the initial portion of each shrinking curve (Fig. 5) in order to measure the time to reach equilibrium volume using the initial, rapid rate of exit. Average values of these times were plotted against external concentrations of penetrants (Fig. 6). These graphs should enable one to distinguish between simple and facilitated diffusion. Least squares regression lines were calculated for each of these graphs to obtain values of the x-intercepts (Table I). DISCUSSION Previous work has shown that glycerol, ethylene glycol, thiourea and urea cross the membrane of erythrocytes of several species of mammals and one species of birds by facilitated diffusion (Hunter, 1970a, b; Cainelli, Chui, McClure and Hunter, 1974; Hunter, 1976; Hunter, ci al, 1976). The present experiments. Please note that these images ar
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
