. Public health laboratory work, including methods employed in bacteriological research, with special reference to the examination of air, water and food contributed . Fig. ioo.—The autoclave, showing flasks in the interior undergoing sterilisation. be allowed to issue through the stop cock for about fiveminutes. When the cock is closed, the guage must beclosely watched, lest any accident should happen. Thedesired temperature having been reached, the gas mustbe lowered, in order to keep the temperature con- STERILISATION. 431 stant. The stop cock nrnst not he opened till the pressure in-side t
. Public health laboratory work, including methods employed in bacteriological research, with special reference to the examination of air, water and food contributed . Fig. ioo.—The autoclave, showing flasks in the interior undergoing sterilisation. be allowed to issue through the stop cock for about fiveminutes. When the cock is closed, the guage must beclosely watched, lest any accident should happen. Thedesired temperature having been reached, the gas mustbe lowered, in order to keep the temperature con- STERILISATION. 431 stant. The stop cock nrnst not he opened till the pressure in-side the boiler has regained the normal. For most purposes an exposure to a temperature of115° C. for twenty minutes, or to 120° for ten to fifteenminutes, serves to sterilise. Gelatine, it is to be re-membered, must not be superheated, so that the stopcock must be left open whilst making it. In the manu-facture of agar, the autoclave is very useful, the high.
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