Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . the ammonia originallypresent in the urine and in the magnesium chloride. V. Folins Method.—Place 2^ of urine in an rerometercylinder, 30-45 cm. in height (Fig i__\ p. 358), add about onegram of dry sodium carbonate and introduce some crudepetroleum to prevent foaming-. Insert into the neck of thecylinder a rubber stopper provided with two perforations intoeach of which passes a glass tube one of which reaches belowthe surface of th
Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . the ammonia originallypresent in the urine and in the magnesium chloride. V. Folins Method.—Place 2^ of urine in an rerometercylinder, 30-45 cm. in height (Fig i__\ p. 358), add about onegram of dry sodium carbonate and introduce some crudepetroleum to prevent foaming-. Insert into the neck of thecylinder a rubber stopper provided with two perforations intoeach of which passes a glass tube one of which reaches belowthe surface of the liquid. The shorter tube (10 cm. in length |is connected with a calcium chloride tube filled with cottonand this tube is in turn joined to a glass tube extendingto the bottom of a 500 wide mouthed ilad< which isintended to absorb the for this purpose shouldcontain 20 of y$ sulphuric acid, 200 of distilled 1 There is some decomposition of urea at 6o° C. 35* PHYSIOLOGICAL CHEMISTRY. water and a few drops of an indicator ( alizarin red ). Toinsure the complete absorption of the ammonia the absorption Fig. Folins Ammonia Apparatus. flask is provided with a Folin absorption tube (Fig. 123, p. 359)which is very effective in causing the air passing from thecylinder to come into intimate contact with the acid in theabsorption flask. In order to exclude any error due to thepresence of ammonia in the air a similar absorption apparatusto the one just described is attached to the other side of theaerometer cylinder, thus insuring the passage of ammonia-freeair into the cylinder. With an ordinary filter pump and goodwater pressure the last trace of ammonia should be removedfrom the cylinder in about one and one-half Thenumber of cubic centimeters of the jq sulphuric acid neutral-ized by the ammonia of the urine may be determined by directtitration with y^ sodium hydroxide. This is one of the most satisfactory methods yet devised forthe determ
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