. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . CULTURE MEDIA 93 raise the upper end. Care should be taken that the slant does not touch thecotton, and sufficient media be present in the bottom of the tube (butt)so that on raising the tube the slant will not fall down. The medium shouldbe well set before Fig. 27 Fig. 28 Fig. 29 Types of filters. Fig. 30 Sterilization of Media.—Sterilization may be accomplished bv filtrationthrough various types of filters which do not allow the passage of bacteria, byheat, and occasionally by chemicals
. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . CULTURE MEDIA 93 raise the upper end. Care should be taken that the slant does not touch thecotton, and sufficient media be present in the bottom of the tube (butt)so that on raising the tube the slant will not fall down. The medium shouldbe well set before Fig. 27 Fig. 28 Fig. 29 Types of filters. Fig. 30 Sterilization of Media.—Sterilization may be accomplished bv filtrationthrough various types of filters which do not allow the passage of bacteria, byheat, and occasionally by chemicals, as chloro-form. Sterilization bj heat may be accom-plished in several ways—namely, intermittent(fractional) steriUzation at 100° C. or at 60°to 80° C, and sterilization by steam underpressure. Filtration. — This method is resorted towhere the application of heat will inj ure thesolution, as in the case of some sugars, orwhere the necessary amount of heat wouldcause coagulation, as in the case of tissueextracts or serum. Various types of filtersmade of unglazed porcelain or compresseddiatomaceous earth are used. Types of thesefilters are the Berkefeld, Pasteur, Chamberland,and Doulton filters. The bacteria are heldback because of the fineness of the grades of fineness are procurable insome brands, and on this depends the rapidityo
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