. Canadian journal of agricultural science. Agricultural Institute of Canada; Agriculture. 264 SCIENTmC AGRICULTUKE. April, 1922. duc-ed to germinate and made to produce healthy plants. CAUTION,—The above method should be carried out strictly in accordance with instructions given. By improper mani- pulation, the germinating power of the hard seed may be completely destroyed. Hardening of Seedling Plants. After germination of the seed and when the seedling plants have got to about the fourth leaf stage, they are transplanted singly into three-inch flower pots. With a moderate greenhouse tempera


. Canadian journal of agricultural science. Agricultural Institute of Canada; Agriculture. 264 SCIENTmC AGRICULTUKE. April, 1922. duc-ed to germinate and made to produce healthy plants. CAUTION,—The above method should be carried out strictly in accordance with instructions given. By improper mani- pulation, the germinating power of the hard seed may be completely destroyed. Hardening of Seedling Plants. After germination of the seed and when the seedling plants have got to about the fourth leaf stage, they are transplanted singly into three-inch flower pots. With a moderate greenhouse temperature, a sturdy growth is encouraged, and finally the plants are transferred to a cold frame to harden off before planting out in the open field. The germination of the seeds and the manipulation of the plants are .so adjusted that plants are usually ready to transplant out in the field from about the middle to the end of May. In the field, the plants are usually spaced from three to four feet apart each way. This allows for the study of the individual plants and also gives the necessary working space between the l)lants. (Fig. VI.) If Alfalfa plants are raised in the man- ner described above, it is usually possible to obtain seeds the same year of planting; and thus the worker is enabled to con- tinue further investigations with a mini- mum of lost time. RefereTices to Literature Cited in Text. 1. Love, H. H. and Leighty. C. E. Germ- ination of Seed as Affected by Sul- phuric Acid Treatment; Cornell Univ., Col. of Agriculture, Dep't of Plant Breeding, Bulletin 312. Mai-ch 1912. 2. Oliver, G. W. New Methods of Plant Breeding; U. S. Dep't of Agr., B. P. I., Bulletin 167, 1910. 3. Piper, C. V. Alfalfa Seed Production; Pollination Studies: U. S. Dep't of Agr., B. P. T. Bulletin 75, April, 1914. 4. South worth, W. Alfalfa Hybridization: Journal of Hereditv, vol. V, No. 10. October. Fig. VI. Second growth of Alfalfa showing method of spacing plants. (Note: The plants selecte


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