. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AN EXPERIMENTAL STUDY OF POLYSPERMY 379 would be strictly comparable with the values obtained for the other agents. This work differed from Smith and Clowes' in that counts were made at the time of the first cleavage instead of 2-4 hours after insemination, and treated eggs were inseminated with a light sus- pension of sperm (see above) instead of the "excess sperm" used by Smith and Clowes. In other respects their technique was followed 100 r. P H FIG. 4. Effect of pH on development and polyspe
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AN EXPERIMENTAL STUDY OF POLYSPERMY 379 would be strictly comparable with the values obtained for the other agents. This work differed from Smith and Clowes' in that counts were made at the time of the first cleavage instead of 2-4 hours after insemination, and treated eggs were inseminated with a light sus- pension of sperm (see above) instead of the "excess sperm" used by Smith and Clowes. In other respects their technique was followed 100 r. P H FIG. 4. Effect of pH on development and polyspermy in Arbacia punctulata. Open circles, development; solid circles, polyspermy. exactly. The sea water buffers were broken down by the addition of 23 cc. HCl/liter of sea water, and the water was allowed to stand overnight in a shallow dish to permit escape of the excess CO2. The pH was adjusted by the addition of NaOH, and checked with Clark and Lubs indicators. Eggs were inseminated in the solutions after 10 minutes exposure. The results, as shown in Table XII, correspond well with those of Smith and Clowes for Arbacia, when allowance is made for the lighter. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
