. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CRAYFISH TESTICULAR MATURATION 343 200 •. TREATMENT Figure 4. Effect of different treatments on the mean testicular index of the crayfish, Procambants clarkii- 1C, initial control; SC, simultaneous control; SP, 1 X 10~6 mol spiperone per crayfish: PI. 1 x 10~6 mol pim- ozide per crayfish; 5-HT, 1 X 10"6 mol 5-HT per crayfish; COMB, com- bination of 1 x 10~6mol DA per crayfish + 1 X 10"6 mol 5-HT per crayfish. Error bars are SEM. Bar SC is significantly (P < ) larger than bar 1C. but bar SC is signi
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CRAYFISH TESTICULAR MATURATION 343 200 •. TREATMENT Figure 4. Effect of different treatments on the mean testicular index of the crayfish, Procambants clarkii- 1C, initial control; SC, simultaneous control; SP, 1 X 10~6 mol spiperone per crayfish: PI. 1 x 10~6 mol pim- ozide per crayfish; 5-HT, 1 X 10"6 mol 5-HT per crayfish; COMB, com- bination of 1 x 10~6mol DA per crayfish + 1 X 10"6 mol 5-HT per crayfish. Error bars are SEM. Bar SC is significantly (P < ) larger than bar 1C. but bar SC is significantly (P < ) smaller than bars SP. PI. 5-HT. and COMB. Bar 5-HT is significantly (P < ) larger than bar COMB. 5-HT COMB TREATMENT Figure 5. Effect of different treatments on the mean testicular lobe diameter of the crayfish. Pnicninhariis clarkii. 1C. initial control; SC. simultaneous control; SP, 1 X ICT'mol spiperone per crayfish; PI, 1 x 10~6 mol pimozide; 5-HT. 1 x 10~6 mol 5-HT per crayfish; COMB, combination of I x 10~6 mol DA per crayfish + 1 X 1CT6 mol 5-HT per crayfish. Error bars are SEM. Bar SC is significantly (P < ) larger than bar 1C. but bar SC is significantly (P < ) smaller than bars SP, PI. 5-HT. and COMB. Bar 5-HT is significantly (P < ) larger than bar COMB. Effects of the DA receptor Mockers spiperone ami pimozide. 5-HT alone, and 5-HT in combination with DA on the testes For each replicate of this set of experiments, 6 groups of 10 crayfish were selected from the stock. One group served as the initial control: the crayfish of this group were treated in the same way as the initial control crayfish of the DA dose-response experiment. The crayfish in the simultaneous control group received physiological saline in 100 jul doses. Two groups received 1 X 10"" mol of the DA receptor blockers spiperone and pimozide respectively in 100 n\ doses. Another group received 1 X 10 6 mol of 5-HT per crayfish in 100-^1 dose
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