. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EXTRA CLAW ON CHELIPED OF CRAYFISH 53 sors. The V-shaped incision was made to a depth of about half the width of the base of the propodus (Fig. 1). The ventro-median tissues were destroyed by inserting a nee- dle (1 mm in diameter) into the wound. In 34 crayfish, a total of 134 chelipeds were wounded. Both eyestalks of five of the crayfish were removed from the base 10 to 20 days post-operation. Rearing wounded crayfish From September 1996 to March 1997, the wounded crayfish were reared until they had molted two or three tim
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EXTRA CLAW ON CHELIPED OF CRAYFISH 53 sors. The V-shaped incision was made to a depth of about half the width of the base of the propodus (Fig. 1). The ventro-median tissues were destroyed by inserting a nee- dle (1 mm in diameter) into the wound. In 34 crayfish, a total of 134 chelipeds were wounded. Both eyestalks of five of the crayfish were removed from the base 10 to 20 days post-operation. Rearing wounded crayfish From September 1996 to March 1997, the wounded crayfish were reared until they had molted two or three times. The animals were kept separately in individual containers (300 X 240 X 105 mm) under a natural photo- period at room temperature (18°-26°C). Prawn pellets (Super B; Nihon Nosan Industry, Japan) and dry persim- mon leaves were placed in the rearing containers so that the crayfish could feed on them ad libitum. Sensory neuron responses A cheliped with extra dactyls was cut off at the base of the merus and held on a glass slide by rubber bands. The exoskeleton at the base of the merus was removed and the nerve bundles were exposed. To detect responses from the sensory neurons, the point of a needle was used to push the extra dactyls in a closing direction. Action potentials from the exposed nerve bundles were recorded extracellularly, using suction electrodes. Mechanical force exerted by closure of dactyls Measurements were taken on the force exerted by clos- ing the dactyls. The cheliped with double extra dactyls was cut off at the base of the merus and prepared as described above. Nerve bundles were stimulated using a pair of tungsten hook electrodes. Repetitive pulses (single pulse, 10 ms in duration; 20 Hz) were applied for s to hook electrodes connected to an electronic stimulator (SEN1101; Nihon Kohden, Japan). The mechanical force exerted by closure of each of the two dactyls was recorded V-shaped wound Dactyl. Carpus Propodus Pollex Figure 1. Diagram of the sec
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
