. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 3. Cytoskeletal variants in the normal unactivated amehocue population; paired images; phase contrast and fluorescence, (a, a') Ame- bocyte, with a circular rather than elliptical profile, and with a circular MB. (b. b') Singly-pointed amebocyte. with microtubule bundles following the entire cell contour including the pointed tip (arrowheads). This variant type was found in every animal examined (see also Table 1). (c. c'; d. d') Examples of doubly-pointed cells, with microtubule bundles forming in- complete, doubly-p
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 3. Cytoskeletal variants in the normal unactivated amehocue population; paired images; phase contrast and fluorescence, (a, a') Ame- bocyte, with a circular rather than elliptical profile, and with a circular MB. (b. b') Singly-pointed amebocyte. with microtubule bundles following the entire cell contour including the pointed tip (arrowheads). This variant type was found in every animal examined (see also Table 1). (c. c'; d. d') Examples of doubly-pointed cells, with microtubule bundles forming in- complete, doubly-pointed MBs (arrowheads). This variant was found only in some animals. Bar = 10 /am. sections, the closest approach of MB component microtu- bules to the plasma membrane was —50 nm, as in the case of granules (Fig. 5d; arrowheads and white bars). Centrioles were found regularly, often with both mem- bers of a pair in a given section, usually located between the nucleus and Golgi apparatus. Centrioles had classic cylin- drical structure. — X jim, with one closed end (Fig. 5f, arrow) and typical 9 + 0 triplet cross section (Fig. 5g). Centriole pairs were present in both parallel and perpendic- ular orientation, and when both members of a pair were ® f. Figure 4. F-actin distribution in unactivated cells; phase contra-.! and fluorescence microscopy, (a) Phase contrast, several cells with nuclei visible, (b) Same cells as in (a), rhodamine-phalloidin binding by F-actin filaments. F-actin was concentrated principally in regions near the nucleus, and in a cortical layer visible in edge view at the cell periphery (b. arrows). Bar = 10 Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 19
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