. A manual of bacteriology for agricultural and general science students. Bacteriology. CULTURE METHODS 31 After inoculating the culture medium, burn over the cotton stoppers and, with forceps sterilized iu the flame, push the stop- pers into the test tubes for a distance of 2 or 3 cm. It is gen- erally desirable to cut off part of the protruding cotton before doing this. Fill the space above the stopper with dry pyro- galUc acid. Add with a pipette enough of a 5 per cent solution of sodium hydrate to dissolve the acid. Close the tubes immediately with a tight-fitting rubber stopper. Invert th
. A manual of bacteriology for agricultural and general science students. Bacteriology. CULTURE METHODS 31 After inoculating the culture medium, burn over the cotton stoppers and, with forceps sterilized iu the flame, push the stop- pers into the test tubes for a distance of 2 or 3 cm. It is gen- erally desirable to cut off part of the protruding cotton before doing this. Fill the space above the stopper with dry pyro- galUc acid. Add with a pipette enough of a 5 per cent solution of sodium hydrate to dissolve the acid. Close the tubes immediately with a tight-fitting rubber stopper. Invert the tube if it contains a sohd medium, and set it aside for Fig. 17. Kipp apparatus arranged for generating hydrogen A, generator; B, wash bottle containing lead nitrate solution; C, wash bottle containing silver nitrate solution 2. Plates and miscellaneous cultures. Place the cultures in a Novy jar (Fig. 16) connected with a hydrogen generator. Pass the hydrogen from the Kipp generator through two wash bottles, one of which contains lead nitrate solution, the other a solution of sUver nitrate. It is well to add another wash bottle contain- ing potassium permanganate. From time to time collect a test tube full of gas from the outlet. Test by holding a burning match at the mouth of the tube. As long as explosion Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Reed, Howard Sprague, 1876-. Boston, New York, Ginn
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