Electrolytes in biological systems, incorporating Electrolytes in biological systems, incorporating papers presented at a symposium at the Marine Biological Laboratory in Woods Hole, Massachusetts, on September 8, 1954 electrolytesinbi00shan Year: 1955 20 hrs. 0 20 hrs. Fig. 3. Influence of aging and of cyanide ( m/I.) on one-way osmosis through isolated frog skin (Rana esciilenta). Buffered Ringer's solution of physiological and | physiological concentration. Average data of 6 experiments each in a and b using two pieces of skin. Further details see text. Vi^o and indicate rela
Electrolytes in biological systems, incorporating Electrolytes in biological systems, incorporating papers presented at a symposium at the Marine Biological Laboratory in Woods Hole, Massachusetts, on September 8, 1954 electrolytesinbi00shan Year: 1955 20 hrs. 0 20 hrs. Fig. 3. Influence of aging and of cyanide ( m/I.) on one-way osmosis through isolated frog skin (Rana esciilenta). Buffered Ringer's solution of physiological and | physiological concentration. Average data of 6 experiments each in a and b using two pieces of skin. Further details see text. Vi^o and indicate relative rate of outward and inward osmosis re- spectively (22). ment was carried out about 25 hours after the first. When the rate of osmosis in the two directions, in terms of percentage volume change, was then plotted on the ordinate against time on the abscissa, graphs were obtained which are shown in the left half of figure 3. A gain of fluid in one compartment of the osmometer is plotted as a positive volume change; the nearly equal loss in the other compartment is plotted as a negative volume change. The first two sets of data for gain and loss of fluid were set equal to 100. All following readings were expressed as percentages of these original values. It can be seen that, up to about the 15th hour, outward osmosis (solid line) is faster than inward os- mosis (dotted line). From then on, the situation is reversed. When a similar experiment was carried out, except with both saline solutions poisoned by cyanide, it was noticed (fig. 3, right half) that the reversal in the rate of out- ward and inward osmosis had already occurred after about 3 hours.
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